In 1983, Sedgewick and Holt published a paper in the Journal of Immunological Methods describing a novel technique for the enumeration of antibody-secreting cells. The new technique was built on the same solid-phase immunoenzymatic principles as the enzyme-linked immunosorbent assay (ELISA): antigen was immobilized to a solid support (plastic dish) to bind antibodies released by cultured splenocytes. Later, in 1983, another article describing
a similar antibody detection technique was published in the same journal by Czerkinsky and colleagues , who coined the name for this assay “enzymelinked immunospot,” or ELISPOT. Later, the original ELISPOT technique was modified in that the solid phase was coated with antibodies (rather than the antigen) to capture antigens (for example, cytokines) released by cultured cells . As modified, reversed ELISPOT has become very popular and appears to be
used more frequently than its predecessor. Some researchers call it “reversed ELISPOT,” whereas most truncated this name to just “ELISPOT” .
Elispot 